Peptide Synthesis Solid Supports

1、Polystyrene Resin

A basic resin is used as a starting material for the preparation of various resins synthesized in combination with chemical and solid phase polypeptides. Electrophilic aromatic substitution reactions generate benzylamines, benzyl chloride, benzyl ethers and other benzyl derivative analogues. Friedel-Crafts acylation reactions to form polymeric ketones. These products can take advantage of further reactions to produce common resin carriers, as well as many with new properties and uses.

2、Chloromethyl Resin (Merrifield Resin)

Merrifield resin is one of the standard supports for the synthesis of peptides by Boc strategy. Originally, the cesium salt of a protected amino acid was anchored to the chloromethyl support via nucleophilic displacement of chlorine. Cleavage is normally effected by treatment with HF or TFMSA or by catalytic transfer hydrogenolysis, alcohols can be released by using diisobutylaluminium hydride or LiBH4. New isomeric trialkoxybenzylamine resins have been developed by coupling phthalimidomethyl-3,5-dimethoxyphenols to this resin followed by subsequent treatment with hydrazine, that allows DCC coupling with the carboxyl function of amino acids or peptides.

3、Wang Resin(Benzyloxybenzyl Alcohol Resin)

Wang resin is probably the most widely used resin in SPOS. As a standard carrier, it can be used for the solid-phase immobilization of acid and phenol for SPOS. Ester bonds can be realized, which have good stability to various reaction conditions, but can be easily removed by mild acid treatment (usually trifluoroacetic acid). For the immobilization of amines, Wang resin can also be easily converted into the solid-phase equivalent of standard carbamate based protective groups by reacting with phosgene or active carbonates (such as carbonyldiimidazole or bis (p-nitrophenyl) - carbonate).

4、2-Chlorotrityl Chloride Resin

A very acid sensitive resin suitable for the synthesis of protected peptide fragments by the Fmoc-strategy. In particular suitable for the preparation of C-terminal prolyl and cysteinyl peptides. 2-Chlorotrityl resin is highly suitable for the synthesis of peptide alcohols. Cleavage is achieved using AcOH/TFE/DCM, 0.5% TFA or HFIP in DCM, or 1-5% TFA in DCM containing 5% TIS. Trityl linkers have certain advantages in comparison to the alkoxybenzyl alcohol, especially in the area of peptide chemistry. The problem of racemization during the loading of the first amino acid is avoided with trityl linkers.

5、Aminomethyl Resin (AM Resin)

This resin can be easily acylated with any appropriate carboxylic acid-containing linker using standard methods of amide bond formation to furnish supports for solid phase organic synthesis.

It is also used as a scavenger resin in solution phase synthesis to remove excess acids, alkylating agents and other electrophiles.

6、Rink Amide-AM Resin

Rink Amide-AM Resin is attached to the aminomethyl resin by a modified Rink amide linker and is an ideal peptide amide Fmoc SPPS tool. From this resin can be treated with 95% TFA, providing high yields and purity of peptide amides.

Rink Amide-MBHA Resin is attached to the MBHA resin by a modified